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Proceedings Paper

STED super-resolution microscopy in Drosophila tissue and in mammalian cells
Author(s): Lana Lau; Yin Loon Lee; Maja Matis; Jeff Axelrod; Tim Stearns; W. E. Moerner
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Paper Abstract

Far-field super-resolution microscopy is a rapidly emerging method that is opening up opportunities for biological imaging beyond the optical diffraction limit. We have implemented a Stimulated Emission Depletion (STED) microscope to image single dye, cell, and tissue samples with 50-80 nm resolution. First, we compare the STED performance imaging single molecules of several common dyes and report a novel STED dye. Then we apply STED to image planar cell polarity protein complexes in intact fixed Drosophila tissue for the first time. Finally, we present a preliminary study of the centrosomal protein Cep164 in mammalian cells. Our images suggest that Cep164 is arranged in a nine-fold symmetric pattern around the centriole, consistent with findings suggested by cryoelectron tomography. Our work demonstrates that STED microscopy can be used for superresolution imaging in intact tissue and provides ultrastructural information in biological samples as an alternative to immuno-electron microscopy.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79101N (11 February 2011); doi: 10.1117/12.881221
Show Author Affiliations
Lana Lau, Stanford Univ. (United States)
Yin Loon Lee, Stanford Univ. (United States)
Maja Matis, Stanford Univ. (United States)
Jeff Axelrod, Stanford Univ. (United States)
Tim Stearns, Stanford Univ. (United States)
W. E. Moerner, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7910:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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