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Proceedings Paper

Topical photosan-mediated photodynamic therapy for DMBA-induced hamster buccal pouch early cancer lesions: an in vivo study
Author(s): Yih-Chih Hsu; Walter Hong-Shong Chang; Junn-Liang Chang; Kuang-Ting Liu; Chun-Pin Chiang; Chung-Ji Liu; Chih-Ping Chen
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Paper Abstract

Oral cancer has becomes the most prominent cancer disease in recent years in Taiwan. The reason is the betel nut chewing habit combing with smoking and alcohol-drinking lifestyle of people results in oral cancer becomes the fastest growth incident cancer amongst other major cancer diseases. In previous studies showed that photosan, haematoporphyrin derivative (HPD), has demonstrated effective PDT results on human head and neck disease studies. To avoid the systemic phototoxic effect of photosan, this study was designed to use a topical photosan-mediated PDT for treatment of DMBA-induced hamster buccal pouch cancerous lesions. DMBA was applied to one of the buccal pouches of hamsters thrice a week for 10 to 12 weeks. Cancerous lesions were induced and proven by histological examination. These DMBA-induced cancerous lesions were used for testing the efficacy of topical photosan-mediated PDT. Before PDT, fluorescence spectroscopy was used to determine when photosan reached its peak level in the lesional epithelial cells after topical application of photosan gel. We found that photosan reached its peak level in cancerous lesions about 13.5 min after topical application of photosan gel. The cancerous lesions in hamsters were then treated with topical photosan-mediated PDT (fluence rate: 600 mW/cm2; light exposure dose 200 J/cm2) using the portable Lumacare 635 nm fiber-guided light device. Visual examination demonstrated that topical photosan-mediated PDT was an applicable treatment modality for DMBA-induced hamster buccal pouch cancerous lesions.

Paper Details

Date Published: 10 February 2011
PDF: 6 pages
Proc. SPIE 7900, Biophotonics and Immune Responses VI, 790004 (10 February 2011); doi: 10.1117/12.879555
Show Author Affiliations
Yih-Chih Hsu, Chung Yuan Christian Univ. (Taiwan)
Walter Hong-Shong Chang, Chung Yuan Christian Univ. (Taiwan)
Junn-Liang Chang, Taoyuan Armed Forces General Hospital (Taiwan)
Kuang-Ting Liu, Taoyuan Armed Forces General Hospital (Taiwan)
Chun-Pin Chiang, National Taiwan Univ. (Taiwan)
Chung-Ji Liu, National Yang-Ming Univ. (Taiwan)
Taiwan Mackay Memorial Hospital (Taiwan)
Nursing and Management College (Taiwan)
Chih-Ping Chen, Taipei Mackay Memorial Hospital (Taiwan)


Published in SPIE Proceedings Vol. 7900:
Biophotonics and Immune Responses VI
Wei R. Chen, Editor(s)

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