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Proceedings Paper

In vivo investigation of the evolution of skin barrier repair after mechanical injury
Author(s): Steven Walston; Melissa Chu; Inseok Seo; Paulo R. Bargo; Nikiforos Kollias
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Paper Abstract

The stratum corneum (SC) serves a primary function of skin barrier and its maintenance is vital for the existence of terrestrial life. Few studies have been performed for evaluation of human SC repair in vivo, non-invasively. In the present study tape stripping was performed on the arms and legs of seven volunteers until all the SC was removed. The injured site and a control adjacent site were measured over a period of 10 days after the injury to assess functionality and repair. Transepidermal water loss (TEWL), tryptophan fluorescence and reflectance confocal microscopy were used to determine permeability of the skin barrier, cell turnover and epidermis morphology, respectively. The results show an exponential rate of recovery for the skin permeability (TEWL) which contrasted with a linear increase in the thickness of the SC as determined by confocal microscopy. Cell turnover increased rapidly immediately after the injury to 2.5 times the levels of the control site, attaining a maximum of 3.5-4 times greater levels after three days and slowly returned to baseline levels after the ten days. Correlation of the cell turnover to the thickness of the viable epidermis was observed and further studies are under way to interpret these results.

Paper Details

Date Published: 17 February 2011
PDF: 5 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830S (17 February 2011); doi: 10.1117/12.879182
Show Author Affiliations
Steven Walston, Johnson & Johnson CPPW (United States)
Univ. of Southern California (United States)
Melissa Chu, Johnson & Johnson CPPW (United States)
Inseok Seo, Johnson & Johnson CPPW (United States)
Paulo R. Bargo, Johnson & Johnson CPPW (United States)
Nikiforos Kollias, Johnson & Johnson CPPW (United States)


Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Bodo E. Knudsen; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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