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Proceedings Paper

Interferometry for topographical diagnostics of RBCs in optical tweezers
Author(s): Ranjeet Kumar; Sarita Saraswati; Chandra Shakher; Dalip S. Mehta
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Paper Abstract

Simultaneous non-invasive trapping and topography of erythrocytes by interferometric fringe projection profilometry has been presented. Sinusoidal fringes were generated with the help of compact Michelson interferometer (CMI) developed by coating a thin reflective layer (~100μm) of Al2O3 on one face of a cubic beam splitter. An external mirror was mounted on XYZ translational stage to control the fringe frequency and orientation. Red He-Ne laser was used to generate the sinusoidal probe -interferogram using CMI to project onto the green-laser (cw) trapped healthy and deceased RBCs separately. Information coded reflected interferograms exhibits characteristic fringe-deviation with respect to probe-pattern for both the healthy and defective RBCs. Fourier transform analysis was adopted to retrieve the phase-map which can be exploited for topography, size determination and refractive-index of RBC. Refractive-index change is directly related with hemoglobin concentration of RBCs at any specific physiological state and hence information about health status and disease progression can be anticipated.

Paper Details

Date Published: 24 November 2010
PDF: 6 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 73760G (24 November 2010); doi: 10.1117/12.878959
Show Author Affiliations
Ranjeet Kumar, Indian Institute of Technology Delhi (India)
Sarita Saraswati, Univ. of Delhi (India)
Chandra Shakher, Indian Institute of Technology Delhi (India)
Dalip S. Mehta, Indian Institute of Technology Delhi (India)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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