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Proceedings Paper

The morphology of apoptosis and necrosis of fat cells after photodynamic treatment at a constant temperature in vitro
Author(s): Irina Yu. Yanina; Tatyana G. Orlova; Valery V. Tuchin; Gregory B. Altshuler
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Paper Abstract

Photodynamic therapy with temperature control is a new approach for treatment of obesity and cellulite. Cell death can occur under the action of various physical, chemical and biological factors. Depending on the inductor, this is apoptosis or necrosis. These two forms of cell death differ on the biochemical and morphological levels. Biochemical changes occur quickly enough and it raises difficulties of their detection. One of the morphological characteristics of apoptosis is a decrease (contraction) of cells, and necrosis - an increase in the size of the cell (swelling). This attribute simply determined visually using a digital microscope. The program was designed using LabVEIW media, which allowed us to develop the software for providing interaction with the measuring and control equipment, data collection, processing and displaying the information and results of calculations and simulations for the individual cells and ensembles of cells, and, in general, to automate process.

Paper Details

Date Published: 17 February 2011
PDF: 7 pages
Proc. SPIE 7887, Mechanisms for Low-Light Therapy VI, 78870X (17 February 2011); doi: 10.1117/12.878754
Show Author Affiliations
Irina Yu. Yanina, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Tatyana G. Orlova, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Valery V. Tuchin, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Russian Federation)
Gregory B. Altshuler, Palomar Medical Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 7887:
Mechanisms for Low-Light Therapy VI
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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