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Proceedings Paper

Developing a toolbox for analysis of warrior wound biopsies: vibrational spectroscopy
Author(s): Nicole J. Crane; Frederick P. O'Brien; Jonathan A. Forsberg; Benjamin K. Potter; Eric A. Elster
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Paper Abstract

The management of modern traumatic war wounds remains a significant challenge for clinicians. This is a reflection of the extensive osseous and soft-tissue damage caused by blasts and high-energy projectiles. The ensuing inflammatory response ultimately dictates the pace of wound healing and tissue regeneration. Consequently, the eventual timing of wound closure or definitive coverage is often subjectively based. Some wounds require an extended period of time to close or fail to remain closed, despite the use and application of novel wound-specific treatment modalities. Aside from impaired wound healing, additional wound complications include wound infection, biofilm formation, and heterotopic ossification (the pathological mineralization of soft tissues). An understanding of the molecular environment of acute wounds throughout the debridement process can provide valuable insight into the mechanisms associated with the eventual wound outcome. The analysis of Raman spectra of ex vivo wound biopsy tissue obtained from serial traumatic wound debridements reveals a decreased 1665 cm-1/1445 cm-1 band area ratio in impaired healing wounds, indicative of an impaired remodeling process, in addition to a decreased 1240 cm-1/1270cm-1. The examination of debrided tissue exhibits mineralization during the early development of heterotopic ossification. Finally, preliminary results suggest that Fourier transform infrared (FT-IR) images of wound effluent may be able to provide early microbiological information about the wound.

Paper Details

Date Published: 16 February 2011
PDF: 14 pages
Proc. SPIE 7895, Optical Biopsy IX, 78950O (16 February 2011); doi: 10.1117/12.877478
Show Author Affiliations
Nicole J. Crane, Naval Medical Research Ctr. (United States)
Frederick P. O'Brien, Walter Reed National Military Medical Ctr. (United States)
Jonathan A. Forsberg, Walter Reed National Military Medical Ctr. (United States)
Uniformed Services Univ. of Health Sciences (United States)
Benjamin K. Potter, Walter Reed National Military Medical Ctr. (United States)
Uniformed Services Univ. of Health Sciences (United States)
Eric A. Elster, Naval Medical Research Ctr. (United States)
Uniformed Services Univ. of Health Sciences (United States)
Walter Reed National Military Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 7895:
Optical Biopsy IX
Robert R. Alfano, Editor(s)

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