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Proceedings Paper

Second harmonic generation imaging of collagen matrix remodeling in a stimulated 3D cellular environment: forward versus backward detection
Author(s): Thomas Abraham; Alex Scott; Jon Carthy; Bruce McManus
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Paper Abstract

The structural remodeling of collagen is important in several biological processes such as wound healing, tendon repair, fibrosis and developmental morphogenesis. Multiphoton microscopy, which uses ultra-short femto-second laser pulses as an excitation source, is efficient in the multiphoton excitation fluorescence (MPEF) of exogenous fluorescent labels tagged to various cellular macromolecular objects, as well as in the induction of a highly specific second harmonic generation (SHG) signal from non-centrosymmetric macromolecules such as fibrillar collagens. Although the non-descanned detectors in the reflection geometry have normally been employed for capturing the backward scattered SHG as well as the MPEF signals, considering the wide range of engineered thick tissue imaging applications, there are still un-answered questions about the generated 3D collagen structures because of the directional pattern of SHG signals. The present study dealt with an in vitro collagen-fibroblast raft model in which the stimulation of fibroblast cells induced the lateral orientation of collagen molecules. The SHG signals originating from the 3D collagen matrix were captured simultaneously in both forward and backward scattering directions to understand the collagen structural differences and to generate a comprehensive understanding of collagen matrix remodeling.

Paper Details

Date Published: 28 February 2011
PDF: 12 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030E (28 February 2011); doi: 10.1117/12.877467
Show Author Affiliations
Thomas Abraham, The Univ. of British Columbia (Canada)
Alex Scott, The Univ. of British Columbia (Canada)
Jon Carthy, The Univ. of British Columbia (Canada)
Bruce McManus, The Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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