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Proceedings Paper

Characterizing collagen-based materials modified by glycation: a multiphoton optical image guided spectroscopy method
Author(s): Yu-Jer Hwang; Joseph Granelli; Christina Flores; Julia Lyubovitsky
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Paper Abstract

In spite of the adverse ageing effects of glycation in vivo, in vitro this process is widely employed to increase stiffness and strength of tissues' and artificial scaffolds'. In-situ optical characterization methods that report on the structures within these materials could clarify the effects of glycation. We employed one-photon fluorescence and multiphoton microscopy method that combined two-photon fluorescence and second harmonic generation signals to characterize collagen hydrogels modified with glyceraldehyde, ribose and glucose. We observed an increase in the in situ fluorescence as well as structural alterations within the materials during the course of glycation.

Paper Details

Date Published: 28 February 2011
PDF: 7 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021I (28 February 2011); doi: 10.1117/12.877112
Show Author Affiliations
Yu-Jer Hwang, Univ. of California, Riverside (United States)
Joseph Granelli, Univ. of California, Riverside (United States)
Christina Flores, Univ. of California, Riverside (United States)
Julia Lyubovitsky, Univ. of California, Riverside (United States)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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