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Proceedings Paper

Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells
Author(s): Huai-na Yu; Ying-ying Lu; Tong-sheng Chen
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Paper Abstract

Dihydroartemisinin (DHA), a first-line anti-malarial drug with low toxicity, has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathway, but the molecular mechanisms are not well understood. In this paper, we focus on whether Bad, a BH3-only pro-apoptotic protein, is involved in apoptotic cell death in DHA-treated human lung adenocarcinoma (ASTC-a-1) cells. Confocal fluorescence microscope imaging was used to monitor the temporal and spatial distribution of Bad in single living cells. Our results indicate that Bad is still located in cytoplasm and does not translocate to mitochondria after treatment with DHA for 24 h, while only a small proportion of Bad located in cytoplasm in the STS-treated cells for 6 h. These results show for the first time that Bad is not involved in DHA-induced apoptosis in ASTC-a-1 cells, which could give more evidence for the molecular mechanisms of apoptosis induced by DHA.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000J (11 February 2011); doi: 10.1117/12.876977
Show Author Affiliations
Huai-na Yu, MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ. (China)
Ying-ying Lu, MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ. (China)
Tong-sheng Chen, MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 7900:
Biophotonics and Immune Responses VI
Wei R. Chen, Editor(s)

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