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Proceedings Paper

Effects of 810 nm laser on mouse primary cortical neurons
Author(s): Gitika B. Kharkwal; Sulbha K. Sharma; Ying-Ying Huang; Luis De Taboada; Thomas McCarthy; Michael R. Hamblin
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Paper Abstract

In the past four decades numerous studies have reported the efficacy of low level light (laser) therapy (LLLT) as a treatment for diverse diseases and injuries. Recent studies have shown that LLLT can biomodulate processes in the central nervous system and has been extensively studied as a stroke treatment. However there is still a lack of knowledge on the effects of LLLT at the cellular level in neurons. The present study aimed to study the effect of 810 nm laser on several cellular processes in primary cortical neurons cultured from mouse embryonic brains. Neurons were irradiated with light dose of 0.03, 0.3, 3, 10 and 30 J/cm2 and intracellular levels of reactive oxygen species, nitric oxide and calcium were measured. The changes in mitochondrial function in response to light were studied in terms of adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP). Light induced a significant increase in calcium, ATP and MMP at lower fluences and a decrease at higher fluence. ROS was induced significantly by light at all light doses. Nitric oxide levels also showed an increase on treatment with light. The results of the present study suggest that LLLT at lower fluences is capable of inducing mediators of cell signaling process which in turn may be responsible for the biomodulatory effects of the low level laser. At higher fluences beneficial mediators are reduced but potentially harmful mediators are increased thus offering an explanation for the biphasic dose response.

Paper Details

Date Published: 17 February 2011
PDF: 8 pages
Proc. SPIE 7887, Mechanisms for Low-Light Therapy VI, 788707 (17 February 2011); doi: 10.1117/12.876664
Show Author Affiliations
Gitika B. Kharkwal, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Sulbha K. Sharma, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Ying-Ying Huang, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Guangxi Medical Univ. (China)
Luis De Taboada, Photothera Inc. (United States)
Thomas McCarthy, Photothera Inc. (United States)
Michael R. Hamblin, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Harvard-MIT Division of Health Sciences and Technology (United States)


Published in SPIE Proceedings Vol. 7887:
Mechanisms for Low-Light Therapy VI
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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