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Proceedings Paper

Mechanisms of gold nanoparticle mediated ultrashort laser cell membrane perforation
Author(s): M. Schomaker; J. Baumgart; D. Motekaitis; D. Heinemann; J. Krawinkel; M. Pangalos; W. Bintig; E. Boulais; R. Lachaine; B. St.-Louis Lalonde; A. Ngezahayo; M. Meunier; A. Heisterkamp
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Paper Abstract

The gold nanoparticle (AuNP) mediated ultrashort laser cell membrane perforation has been proven as an efficient delivery method to bring membrane impermeable molecules into the cytoplasm. Nevertheless, the underlying mechanisms have not been fully determined yet. Different effects may occur when irradiating a AuNP with ultrashort laser pulses and finally enable the molecule to transfer. Depending on the parameters (pulse length, laser fluence and wavelength, particle size and shape, etc.) light absorption or an enhanced near field scattering can lead to perforation of the cell membrane when the particle is in close vicinity. Here we present our experimental results to clarify the perforation initiating mechanisms. The generation of cavitation and gas bubbles due to the laser induced effects were observed via time resolved imaging. Additionally, pump-probe experiments for bubble detection was performed. Furthermore, in our patch clamp studies a depolarization of the membrane potential and the current through the membrane of AuNP loaded cell during laser treatment was detected. This indicates an exchange of extra- and intra cellular ions trough the perforated cell membrane for some milliseconds. Additionally investigations by ESEM imaging were applied to study the interaction of cells and AuNP after co incubation. The images show an attachment of AuNP at the cell membrane after several hours of incubation. Moreover, images of irradiated and AuNP loaded cells were taken to visualize the laser induced effects.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7925, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI, 79250F (11 February 2011); doi: 10.1117/12.876625
Show Author Affiliations
M. Schomaker, Laser Zentrum Hannover e.V. (Germany)
J. Baumgart, École Polytechnique de Montréal (Canada)
D. Motekaitis, Laser Zentrum Hannover e.V. (Germany)
D. Heinemann, Laser Zentrum Hannover e.V. (Germany)
J. Krawinkel, Laser Zentrum Hannover e.V. (Germany)
M. Pangalos, Leibniz Univ. Hannover (Germany)
W. Bintig, Leibniz Univ. Hannover (Germany)
E. Boulais, École Polytechnique de Montréal (Canada)
R. Lachaine, École Polytechnique de Montréal (Canada)
B. St.-Louis Lalonde, École Polytechnique de Montréal (Canada)
A. Ngezahayo, Leibniz Univ. Hannover (Germany)
M. Meunier, École Polytechnique de Montréal (Canada)
A. Heisterkamp, Laser Zentrum Hannover e.V. (Germany)


Published in SPIE Proceedings Vol. 7925:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI
Alexander Heisterkamp; Joseph Neev; Stefan Nolte, Editor(s)

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