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Proceedings Paper

Adaptive optics confocal fluorescence microscopy with direct wavefront sensing for brain tissue imaging
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Paper Abstract

Recently, there has been a growing interest in deep tissue imaging for the study of neurons. Unfortunately, because of the inhomogeneous refractive index of the tissue, the aberrations degrade the resolution and brightness of the final image. In this paper, we describe an adaptive optics confocal fluorescence microscope (AOCFM) which can correct aberrations based on direct wavefront measurements using a point source reference beacon and a Shack-Hartmann Wavefront Sensor (SHWS). Mouse brain tissues with different thicknesses are tested. After correction, both the signal intensity and contrast of the image are improved.

Paper Details

Date Published: 26 March 2011
PDF: 8 pages
Proc. SPIE 7931, MEMS Adaptive Optics V, 79310L (26 March 2011); doi: 10.1117/12.876524
Show Author Affiliations
Xiaodong Tao, Univ. of California, Santa Cruz (United States)
Bautista Fernandez, Univ. of California, Santa Cruz (United States)
Diana C. Chen, Lawrence Livermore National Lab. (United States)
Oscar Azucena, Univ. of California, Santa Cruz (United States)
Min Fu, Univ. of California, Santa Cruz (United States)
Yi Zuo, Univ. of California, Santa Cruz (United States)
Joel Kubby, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 7931:
MEMS Adaptive Optics V
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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