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Proceedings Paper

Fourier analysis of mitochondrial distribution in oocytes
Author(s): Joseph L. Hollmann; Dana H. Brooks; Judith A. Newmark; Carol M. Warner; Charles A. DiMarzio
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Paper Abstract

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

Paper Details

Date Published: 23 February 2011
PDF: 8 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790419 (23 February 2011); doi: 10.1117/12.876345
Show Author Affiliations
Joseph L. Hollmann, Northeastern Univ. (United States)
Dana H. Brooks, Northeastern Univ. (United States)
Judith A. Newmark, Northeastern Univ. (United States)
CompuCyte (United States)
Carol M. Warner, Northeastern Univ. (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)

Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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