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Proceedings Paper

Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors
Author(s): Chunfeng Zhang; Justin Ingram; Steven Schiff; Jian Xu; Min Xiao
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Paper Abstract

We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.

Paper Details

Date Published: 1 March 2011
PDF: 6 pages
Proc. SPIE 7947, Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII, 79470B (1 March 2011); doi: 10.1117/12.876341
Show Author Affiliations
Chunfeng Zhang, Nanjing Univ. (China)
The Pennsylvania State Univ. (United States)
Justin Ingram, The Pennsylvania State Univ. (United States)
Steven Schiff, The Pennsylvania State Univ. (United States)
Jian Xu, The Pennsylvania State Univ. (United States)
Min Xiao, Nanjing Univ. (China)


Published in SPIE Proceedings Vol. 7947:
Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII
Kurt G. Eyink; Frank Szmulowicz; Diana L. Huffaker, Editor(s)

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