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Proceedings Paper

Arrhenius parameters for primary thermal injury in human tonsillar tissue
Author(s): Kathleen McMillan; Rebecca Radabaugh; James E. Coad
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Paper Abstract

Clinical implementation of a thermal therapy requires the ability to predict tissue injury following exposures to specific thermal histories. As part of an effort to develop a nonexcisional alternative to tonsillectomy, the degree of primary hyperthermic tissue injury in human tonsil was characterized. Fifteen fresh pediatric hypertrophic tonsillectomy specimens were sectioned and treated in a NIST-calibrated saline bath at temperatures of 40 to 70°C with hold times of one to seven minutes. The treated tissues were subsequently nitroblue tetrazolium (NBT) stained to assess for thermal respiratory enzyme inactivation as a marker of cellular injury/death. The NBT stains were quantitatively image analyzed and used to calculate Arrhenius parameters for primary thermal injury in human tonsils.

Paper Details

Date Published: 22 February 2011
PDF: 13 pages
Proc. SPIE 7901, Energy-based Treatment of Tissue and Assessment VI, 790110 (22 February 2011); doi: 10.1117/12.876338
Show Author Affiliations
Kathleen McMillan, gRadiant Research, LLC (United States)
Rebecca Radabaugh, West Virginia Univ. (United States)
James E. Coad, West Virginia Univ. (United States)


Published in SPIE Proceedings Vol. 7901:
Energy-based Treatment of Tissue and Assessment VI
Thomas P. Ryan, Editor(s)

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