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Proceedings Paper

Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells
Author(s): Pei-Hua Chung; James A. Levitt; Marina K. Kuimova; Gokhan Yahioglu; Klaus Suhling
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Paper Abstract

Meso-substituted boron-dipyrromethene (BODIPY-C12) was used to monitor the viscosity in cells via fluorescence lifetime imaging (FLIM), and time-resolved fluorescence anisotropy measurements. Our results show that meso-substituted BODIPY-C12 senses the viscosity in HeLa cells and is insensitive to the surrounding polarity. The relationship between the fluorescence lifetime and the rotational correlation time of the dye in homogeneous solutions agree with the combination of the Foerster Hoffmann equation and the Debye-Stokes-Einstein equation.

Paper Details

Date Published: 22 February 2011
PDF: 10 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790323 (22 February 2011); doi: 10.1117/12.876257
Show Author Affiliations
Pei-Hua Chung, King's College London (United Kingdom)
James A. Levitt, King's College London (United Kingdom)
Marina K. Kuimova, Imperial College London (United Kingdom)
Gokhan Yahioglu, Imperial College London (United Kingdom)
PhotoBiotics Ltd. (United Kingdom)
Klaus Suhling, King's College London (United Kingdom)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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