Share Email Print
cover

Proceedings Paper

Wide-field two-photon microscopy with temporal focusing and HiLo background rejection
Author(s): Elijah Y. S. Yew; Heejin Choi; Daekeun Kim; Peter T. C. So
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging system. Because it is a widefield technique, the optical sectioning effect is considerably poorer than with conventional spatial focusing two-photon microscopy. Here we propose wide-field two-photon microscopy based on spatio-temporal focusing and employing background rejection based on the HiLo microscope principle. We demonstrate the effects of applying HiLo microscopy to widefield temporally focused two-photon microscopy.

Paper Details

Date Published: 28 February 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031O (28 February 2011); doi: 10.1117/12.876068
Show Author Affiliations
Elijah Y. S. Yew, Singapore-MIT Alliance for Research and Technology (Singapore)
Heejin Choi, Massachusetts Institute of Technology (United States)
Daekeun Kim, Massachusetts Institute of Technology (United States)
Peter T. C. So, Singapore-MIT Alliance for Research and Technology (Singapore)
Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top