Share Email Print
cover

Proceedings Paper

Development of a stigmatic imaging mass spectrometer using laser desorption/ionization
Author(s): Kunio Awazu; Hisanao Hazama; Hirofumi Nagao; Hidetoshi Yoshimura; Jun Aoki; Kenichi Fujii; Katsuyoshi Masuda; Toshio Tashima; Michisato Toyoda; Yasuhide Naito
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Imaging mass spectrometry based on matrix-assisted laser desorption/ionization gives distributions of multiple biomolecules and identification of analytes in a tissue section. We are developing a stigmatic imaging mass spectrometer using a multi-turn time-of-flight mass spectrometer, MULTUM-IMG. A mesh pattern image was obtained with spatial resolution of 3 μm. Separation of stigmatic ion images according to the mass-to-charge ratio was also successfully demonstrated with the micro-dot pattern made with crystal violet and methylene blue. An ion image of a micro-dot pattern sample was observed after ten cycles in the multi-turn circuit of MULTUM-IMG, and the pattern of the sample was maintained. Then, stigmatic ion images of the ions of crystal violet and methylene blue produced from the stained section of a brain were observed. An ion image of whole part of a hippocampus (3.25 mm × 1.5 mm) in the brain section can be obtained within 13 minutes.

Paper Details

Date Published: 23 February 2011
PDF: 6 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790225 (23 February 2011); doi: 10.1117/12.876005
Show Author Affiliations
Kunio Awazu, Osaka Univ. (Japan)
Univ. of Fukui (Japan)
Kyoto Univ. (Japan)
Hisanao Hazama, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Hirofumi Nagao, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Hidetoshi Yoshimura, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Jun Aoki, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Kenichi Fujii, Osaka Institute of Technology (Japan)
Japan Science and Technology Agency (Japan)
Katsuyoshi Masuda, Suntory Institute for Bioorganic Research (Japan)
Japan Science and Technology Agency (Japan)
Toshio Tashima, Japan Science and Technology Agency (Japan)
Michisato Toyoda, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Yasuhide Naito, Graduate School for the Creation of New Photonics Industries (Japan)
Japan Science and Technology Agency (Japan)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top