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Proceedings Paper

Combined two-photon microscopy and optical coherence tomography using individually optimized sources
Author(s): Bosu Jeong; Byunghak Lee; Min Seong Jang; Hyoseok Nam; Hae Koo Kim; Sang June Yoon; Junsang Doh; Sang-Joon Lee; Bo-Gie Yang; Myoung Ho Jang; Ki Hean Kim
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Paper Abstract

Two-photon microscopy (TPM) and optical coherence tomography (OCT) are 3D tissue imaging techniques based on different contrast mechanisms. We developed a combined system of TPM and OCT to provide information of both imaging modalities for in-vivo tissue study. TPM and OCT were implemented by using separate light sources, a Ti-Sapphire laser and a wavelength-swept source centered at 1300 nm respectively, and scanners. Light from the two sources was combined for the simultaneous imaging of tissue samples. TPM provided molecular, cellular information of tissues in the region of a few hundred microns on one side at a sub-cellular resolution, and ran at approximately 40 frames per second. OCT provided structural information in the tissue region larger than TPM images at a sub-tenth micron resolution by using 0.1 numerical aperture. OCT had the field of view of 800 um × 800 um based on a 20x objective, the sensitivity of 97dB, and the imaging speed of 0.8 volumes per second. This combined system was tested with simple microsphere specimens, and then was applied to image the explanted intestine of a mouse model and the plant leaves. Morphology and micro-structures of the intestine villi and immune cells within the villi were shown in the intestine image, and chloroplasts and various microstructures of the maize leaves were visualized in 3D by the combined system.

Paper Details

Date Published: 1 March 2011
PDF: 7 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030Q (1 March 2011); doi: 10.1117/12.876000
Show Author Affiliations
Bosu Jeong, Pohang Univ. of Science and Technology (Korea, Republic of)
Byunghak Lee, Pohang Univ. of Science and Technology (Korea, Republic of)
Min Seong Jang, Pohang Univ. of Science and Technology (Korea, Republic of)
Hyoseok Nam, Pohang Univ. of Science and Technology (Korea, Republic of)
Hae Koo Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
Sang June Yoon, Pohang Univ. of Science and Technology (Korea, Republic of)
Junsang Doh, Pohang Univ. of Science and Technology (Korea, Republic of)
Sang-Joon Lee, Pohang Univ. of Science and Technology (Korea, Republic of)
Bo-Gie Yang, Pohang Univ. of Science and Technology (Korea, Republic of)
Myoung Ho Jang, Pohang Univ. of Science and Technology (Korea, Republic of)
Osaka Univ. (Japan)
Ki Hean Kim, Pohang Univ. of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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