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Proceedings Paper

Live atomic force microscopy imaging of laser microbeam assisted cellular microsurgery
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Paper Abstract

Ultrafast laser microbeam is finding growing usage in causing highly localized damage to cellular structures. This has specifically enhanced efficiency of optoporation-injection of exogenous impermeable substances into the cell by transient pore formation. However, kinetics of laser microbeam induced pore formation and sealing of membrane has not been visualized at nanoscale resolution. Here, we report realization of live atomic force microscopy (AFM) imaging of ultrafast tunable Ti: Sapphire laser microbeam assisted cellular microsurgery. AFM imaging was carried out using Nanonics Multiview system in parallel to exposure of the laser beam. Red blood cells (RBCs) were chosen as cellular model for micro-surgery due to their smooth surface topography. The transparent nature of the Nanonics fiber-optic AFM cantilever allowed simultaneous bright field/phase contrast imaging of the RBC. Measurement of pore size by AFM revealed true pore size as a function of laser exposure duration in contrast to phase contrast imaging. Further, AFM imaging of live cells showed fine topography of sealed pores that could not be comprehended from conventional microscopy.

Paper Details

Date Published: 11 February 2011
PDF: 7 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020L (11 February 2011); doi: 10.1117/12.875985
Show Author Affiliations
Ninad Ingle, The Univ. of Texas at Arlington (United States)
Samarendra K. Mohanty, The Univ. of Texas at Arlington (United States)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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