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Proceedings Paper

Three-dimensional data acquisition with aberrations correction for video-rate microscopy
Author(s): Masood Samim; Richard Cisek; Daaf Sandkuijl; Ian Tretyakov; Sam Siu; Sergey Musikhin; Virginijus Barzda
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Paper Abstract

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of ~200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

Paper Details

Date Published: 23 February 2011
PDF: 7 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790413 (23 February 2011); doi: 10.1117/12.875907
Show Author Affiliations
Masood Samim, Univ. of Toronto (Canada)
Richard Cisek, Univ. of Toronto (Canada)
Daaf Sandkuijl, Univ. of Toronto (Canada)
Ian Tretyakov, Univ. of Toronto (Canada)
Sam Siu, Univ. of Toronto (Canada)
Sergey Musikhin, Univ. of Toronto (Canada)
Virginijus Barzda, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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