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Proceedings Paper

A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging
Author(s): Delong Zhang; Mikhail N. Slipchenko; Shuhua Yue; Junjie Li; Ji-Xin Cheng
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Paper Abstract

We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d31 inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 μm wavelength range, photodamage of biological samples was not observed.

Paper Details

Date Published: 28 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032L (28 February 2011); doi: 10.1117/12.875886
Show Author Affiliations
Delong Zhang, Purdue Univ. (United States)
Mikhail N. Slipchenko, Purdue Univ. (United States)
Shuhua Yue, Purdue Univ. (United States)
Junjie Li, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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