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Proceedings Paper

Determination of cellular injury and death thresholds following exposure to high voltage 10ns electrical pulses
Author(s): Bennett L. Ibey; Caleb C. Roth; Joshua A Bernhard; Andrei G. Pakhomov; Gerald J. Wilmink; Olga Pakhomova
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Paper Abstract

Intense, nanosecond-duration electric pulses (nsEP) have been introduced as a novel modality to alter cellular function, with a mechanism of action qualitatively different from micro- and millisecond duration pulses used in electroporation. In this study, we determined the thresholds for plasma membrane injury (within 15 minutes) and cell death (at 24 hours) for 4 different cell types (CHO-K1, HeLa, Jurkat and U937). Plasma membrane injury was measured by flow cytometry using two fluorescent dyes, namely Annexin V-FITC, which binds to phosphatidylserine (PS) upon its externalization (subtle membrane injury), and propidium iodide (PI), which is typically impermeable to the cell, but enters when large pores are formed in the plasma membrane. In all cell types, 10-ns pulses caused phosphatidylserine (PS) externalization at low doses (<150kV/cm and 100 pulses for each cell type) and no PI uptake. Jurkat and U937 cell lines showed substantial cell death without uptake of PI (15 minutes post exposure) suggesting either delayed permeabilization due to swelling, or damage to intracellular components. In CHO-K1 and HeLa cell lines, PI uptake occurred at low doses relative to that necessary to cause cell death suggesting a necrotic death similar to longer pulse exposures. These findings suggest that nanosecond pulses may be beneficial in applications that require selective elimination of specific cell types.

Paper Details

Date Published: 22 February 2011
PDF: 8 pages
Proc. SPIE 7901, Energy-based Treatment of Tissue and Assessment VI, 79010G (22 February 2011); doi: 10.1117/12.875826
Show Author Affiliations
Bennett L. Ibey, Air Force Research Lab. (United States)
Caleb C. Roth, General Dynamics Information Technology (United States)
Joshua A Bernhard, Air Force Research Lab. (United States)
Andrei G. Pakhomov, Old Dominion Univ. (United States)
Gerald J. Wilmink, Air Force Research Lab. (United States)
Olga Pakhomova, Old Dominion Univ. (United States)

Published in SPIE Proceedings Vol. 7901:
Energy-based Treatment of Tissue and Assessment VI
Thomas P. Ryan, Editor(s)

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