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Proceedings Paper

Nanopore formation in neuroblastoma cells following ultrashort electric pulse exposure
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Paper Abstract

Ultrashort or nanosecond electrical pulses (USEP) cause repairable damage to the plasma membranes of cells through formation of nanopores. These nanopores are able to pass small ions such as sodium, calcium, and potassium, but remain impermeable to larger molecules like trypan blue and propidium iodide. What remains uncertain is whether generation of nanopores by ultrashort electrical pulses can inhibit action potentials in excitable cells. In this paper, we explored the sensitivity of excitable cells to USEP using Calcium Green AM 1 ester fluorescence to measure calcium uptake indicative of nanopore formation in the plasma membrane. We determined the threshold for nanopore formation in neuroblastoma cells for three pulse parameters (amplitude, pulse width, and pulse number). Measurement of such thresholds will guide future studies to determine if USEP can inhibit action potentials without causing irreversible membrane damage.

Paper Details

Date Published: 18 February 2011
PDF: 7 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788354 (18 February 2011); doi: 10.1117/12.875777
Show Author Affiliations
Caleb C Roth, General Dynamics Information Technology (United States)
Jason A Payne, Air Force Research Lab. (United States)
Gerald J. Wilmink, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)


Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Bodo E. Knudsen; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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