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Proceedings Paper

Imaging of the intact mouse cochlea by spectral domain optical coherence tomography
Author(s): Simon S. Gao; Tao Yuan; Anping Xia; Patrick Raphael; Ryan L. Shelton; Brian E. Applegate; John S. Oghalai
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Paper Abstract

Current medical imaging modalities, such as MRI and CT, do not provide high enough resolution to detect many changes within the cochlea that cause hearing loss. We sought to develop the technique of optical coherence tomography (OCT) to image the cochlea noninvasively and within its native environment. We used spectral domain OCT with 950 nm as the center wavelength and a bandwidth of ~100 nm to image freshly excised normal mouse cochlea at different developmental ages. The OCT system has an axial resolution of ~4 μm (in air) and a lateral resolution of ~10 μm. When we imaged normal adult mouse cochleae through the round window membrane, Reissner's membrane, the basilar membrane, the tectorial membrane, the spiral ligament, the spiral limbus, and the modiolus could be clearly identified. When we imaged intact adult cochleae, we were able to image through ~130 μm of bone and tissue to see up to a depth of ~600 μm, and all of the previously identified structures were still visible. Imaging of early postnatal mice during the timeline of cochlear development permitted visualization of the expected structural differences from adult cochleae. Therefore, we conclude that spectral domain OCT is an effective technique for noninvasive imaging of the murine cochlea.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 788931 (11 February 2011); doi: 10.1117/12.875739
Show Author Affiliations
Simon S. Gao, Rice Univ. (United States)
Tao Yuan, Baylor College of Medicine (United States)
Anping Xia, Stanford Univ. (United States)
Patrick Raphael, Stanford Univ. (United States)
Ryan L. Shelton, Texas A&M Univ. (United States)
Brian E. Applegate, Texas A&M Univ. (United States)
John S. Oghalai, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7889:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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