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Proceedings Paper

Automated optical cell detection, sorting, and temperature measurements
Author(s): Joel Kindt; Mujahid Naqbi; Torsten Kiljan; Wesley Fuller; Weina Wang; David W. Kisker; Kevin L. Lear
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Paper Abstract

An automated cell detection and sorting system was developed, combining both the optofluidic intracavity spectroscopy (OFIS) technique and dielectrophoresis (DEP). The OFIS method utilizes a microfluidic channel as a Fabry-Perot cavity to produce characteristic transmission spectra of individual cells. The concept behind optical detection is that a decrease in spectral intensity beyond a threshold indicates that a cell is present. Upon detection, an RF voltage is automatically applied to electrodes, trapping the cell with DEP forces. The system then sorts the cell into one of two microfluidic channels based on resulting optical analysis. A further advantage is that RF joule heating can be measured from known dn/dT values of the medium, which is useful for investigating cell viability issues.

Paper Details

Date Published: 23 February 2011
PDF: 8 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790222 (23 February 2011); doi: 10.1117/12.875608
Show Author Affiliations
Joel Kindt, Colorado State Univ. (United States)
Mujahid Naqbi, Colorado State Univ. (United States)
Torsten Kiljan, Colorado State Univ. (United States)
Wesley Fuller, Colorado State Univ. (United States)
Weina Wang, Colorado State Univ. (United States)
David W. Kisker, eOptra LLC (United States)
Kevin L. Lear, Colorado State Univ. (United States)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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