Share Email Print
cover

Proceedings Paper

Quantitative analysis of diseased horse tendons using Fourier-transform-second-harmonic generation imaging
Author(s): Mayandi Sivaguru; Sushmitha Durgam; Raghu Ambekar; David Luedtke; Glenn Fried; Allison Stewart; Kimani C. Toussaint
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fourier transform-second-harmonic generation (FT-SHG) imaging is used to quantitatively assess the structural organization of collagen fibers in tendonitis-induced horse tendons. Fiber orientation, isotropy, and the ratio of forward to backward SHG signal (F/B ratio) are used to differentiate the fiber organization between the normal and diseased horse tendons. Each second-harmonic generation (SHG) image is divided into several smaller regions of interest (ROI) and the aforementioned quantitative metrics are calculated across the whole grid. ROIs are further labeled as dark (no or minimal presence of fibers), isotropic (random fiber organization), or anisotropic (regular fiber organization) regions. Results show that the normal tendon possesses minimal isotropic regions and small standard deviations in the histograms of orientation and F/B ratio, indicating an intact and highly regular fiber organization. However, the tendonitis-induced horse tendons possess higher number of dark and isotropic regions, and larger standard deviations of the measured parameters, suggesting significantly disoriented and disorganized collagen fibers. This type of quantification would be highly beneficial in diagnosing and determining the stage of tendonitis in clinical settings. Not limited to tendonitis, the technique could also be applied to other diseases that structurally affect collagen fibers. The advantage of FT-SHG over the conventional polarization microscopy is also discussed.

Paper Details

Date Published: 11 February 2011
PDF: 9 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030J (11 February 2011); doi: 10.1117/12.875586
Show Author Affiliations
Mayandi Sivaguru, Univ. of Illinois at Urbana-Champaign (United States)
Sushmitha Durgam, Univ. of Illinois at Urbana-Champaign (United States)
Raghu Ambekar, Univ. of Illinois at Urbana-Champaign (United States)
David Luedtke, Univ. of Illinois at Urbana-Champaign (United States)
Glenn Fried, Univ. of Illinois at Urbana-Champaign (United States)
Allison Stewart, Univ. of Illinois at Urbana-Champaign (United States)
Kimani C. Toussaint, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top