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Proceedings Paper

Characterization of hyperspectral imaging and analysis via microarray printing of dyes
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Paper Abstract

The application of hyperspectral imaging requires rigorous characterization of the spatial and spectral imaging domains of the system. We present a microarray printing methodology for the testing of absorption or reflectance microscopy measurements. This controlled system can serve as a platform for inter-system calibration and provides a common framework for the development of post-processing algorithms. Calibration of the illumination at the objective plane using a transfer standard spectroradiometer allows comparison of light levels regardless of the illumination used, different apertures, and different microscopes. The method uses standard commercial optomechanical components. Printed dyes enable multiplexed testing of the spectral capability of a hyperspectral instrument. The spectral signatures of individual or blended dyes can be analyzed and applied to the testing of spectral image processing tools. Customized programming of the microarrayer allows for arbitrary patterning of dye samples onto the substrate, allowing for the testing of image processing algorithms involving the spatial distribution of spectral features.

Paper Details

Date Published: 22 February 2011
PDF: 8 pages
Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910W (22 February 2011); doi: 10.1117/12.875521
Show Author Affiliations
Matthew L. Clarke, National Institute of Standards and Technology (United States)
Maritoni Litorja, National Institute of Standards and Technology (United States)
David W. Allen, National Institute of Standards and Technology (United States)
Daniel V. Samarov, National Institute of Standards and Technology (United States)
Jeeseong Hwang, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 7891:
Design and Quality for Biomedical Technologies IV
Ramesh Raghavachari; Rongguang Liang, Editor(s)

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