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Proceedings Paper

Multi-target photoacoustic molecular imaging of cardiovascular inflammatory biomarkers using bioconjugated gold nanorods
Author(s): S. Ha; S. Tripathy; A. Carson; L. L. Lavery; H. Zhang; A. Agarwal; N. Kotov; F. S. Villanueva; K. Kim
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Paper Abstract

Multiple cardiovascular inflammatory biomarkers were simultaneously imaged in vivo using antibody conjugated gold nanorods (GNRs) injected into a mouse model of vascular injury stimulated by a photochemical reaction of Rose Bengal dye to green light. Mixed solutions of ICAM-1 antibody conjugated GNRs (715 nm) and E-selectin antibody conjugated GNRs (800 nm) were injected to bind to their respective inflammatory markers on the luminal surface of the inferior vena cava of a mouse. Photoacoustic intensity was measured by a commercial ultrasound probe synchronized to a pulsed laser (10-18 mJ/cm2) at 715 nm or 800 nm clearly identified the upregulation of targeted biomarkers. Histopathology on the harvested tissues confirmed inflammation. The feasibility of simultaneous photoacoustic molecular imaging of inflammation responses in cardiovascular system using a commercial ultrasound system has been demonstrated in vivo.

Paper Details

Date Published: 18 February 2011
PDF: 7 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991M (18 February 2011); doi: 10.1117/12.875459
Show Author Affiliations
S. Ha, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
S. Tripathy, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
A. Carson, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
L. L. Lavery, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
H. Zhang, Univ. of Michigan (United States)
A. Agarwal, Univ. of Michigan (United States)
N. Kotov, Univ. of Michigan (United States)
F. S. Villanueva, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
K. Kim, Univ. of Pittsburgh (United States)
Univ. of Pittsburgh Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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