Share Email Print
cover

Proceedings Paper

Cellular spectroscopy: applications to cancer stem cell characterization
Author(s): G. Wiegand; H. Xin; A. Anderson; J. Mullinax; K. Jaiswal; A. Wiegand; Itzhak Avital
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Spectroscopic and light scattering methods were used to gain insight into the existence and characterization of the cancer stem cell. Fundamental technical description of devices used have been reported elsewhere. We included alterations and implementation of these biophotonic instruments as applied to our objectives. We disassociated human tumor and submitted the cells to optical characterization to support our working hypothesis of stem cell origins to cancer and mechanisms. Single cell combined with population based analysis within the Pancreatic cancer system led us to information regarding the polarization state of cells possessing anchor proteins and drug influx pumps. Multispectral imaging combined with flow cytometry enabled us to target rare cells that appear to retain template DNA. rendering them resistant to anti-cancer drug therapy. In this study we describe an optical method that combines high-throughput population pattern and correlates each cell with an individual fluorescent and bright-field image.

Paper Details

Date Published: 11 February 2011
PDF: 7 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021G (11 February 2011); doi: 10.1117/12.875458
Show Author Affiliations
G. Wiegand, NCI, National Institutes of Health (United States)
H. Xin, NCI, National Institutes of Health (United States)
A. Anderson, NCI, National Institutes of Health (United States)
J. Mullinax, NCI, National Institutes of Health (United States)
K. Jaiswal, NCI, National Institutes of Health (United States)
A. Wiegand, Estuary Biophotonics, Inc. (United States)
Itzhak Avital, NCI, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top