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Proceedings Paper

Multispectral line confocal imaging microscope for fluorescence applications
Author(s): Mark M. Meyers; Iza Ferreira; Pavel Fomitchov; Robert Filkins
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Paper Abstract

We present a novel line confocal microscope for investigating biological samples that simultaneously acquires images from three fluorescent channels. This instrument provides the majority of the benefits of confocal microscopes, such as depth sectioning and improved contrast, while allowing more of the excitation illumination to reach the sample area, resulting in reduced exposure times. One configuration of the line confocal fluorescent microscope is designed to work with a four laser illuminator, and three discrete cameras, while an alternate configuration allows the instrument to act as a multispectral microscope that captures the fluorescent emission over the visible and NIR wavelength range.

Paper Details

Date Published: 11 February 2011
PDF: 11 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020I (11 February 2011); doi: 10.1117/12.875447
Show Author Affiliations
Mark M. Meyers, GE Global Research (United States)
Iza Ferreira, GE Global Research (United States)
Pavel Fomitchov, GE Healthcare Lifesciences (United States)
Robert Filkins, GE Global Research (United States)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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