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Proceedings Paper

Vibrational spectroscopy characterization of low level laser therapy on mammary culture cells: a micro-FTIR study
Author(s): Taciana D. Magrini; Nathalia Villa dos Santos; Marcella Pecora Milazzotto; Giselle Cerchiaro; Herculano da Silva Martinho
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Paper Abstract

Low level laser therapy (LLLT) is an emerging therapeutic approach for several clinical conditions. The clinical effects induced by LLLT presumably go from the photobiostimulation/photobioinibition at cellular level to the molecular level. The detailed mechanism underlying this effect is still obscure. This work is dedicated to quantify some relevant aspects of LLLT related to molecular and cellular variations. This goal was attached by exposing malignant breast cells (MCF7) to spatially filtered light of a He-Ne laser (633 nm) with 28.8 mJ/cm2 of fluency. The cell viability was evaluated by microscopic observation using Trypan Blue viability test. The vibrational spectra of each experimental group (micro- FTIR technique) were used to identify the relevant biochemical alterations occurred due the process. The red light had influence over RNA, phosphate and serine/threonine/tyrosine bands. Light effects on cell number or viability were not detected. However, the irradiation had direct influence on metabolic activity of cells.

Paper Details

Date Published: 18 February 2011
PDF: 7 pages
Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970T (18 February 2011); doi: 10.1117/12.875445
Show Author Affiliations
Taciana D. Magrini, Univ. Federal do ABC (Brazil)
Nathalia Villa dos Santos, Univ. Federal do ABC (Brazil)
Marcella Pecora Milazzotto, Univ. Federal do ABC (Brazil)
Giselle Cerchiaro, Univ. Federal do ABC (Brazil)
Herculano da Silva Martinho, Univ. Federal do ABC (Brazil)

Published in SPIE Proceedings Vol. 7897:
Optical Interactions with Tissue and Cells XXII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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