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Proceedings Paper

Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre
Author(s): Donald A. Peyrot; Claire Lefort; D. Abi Haidar; Tigran Mansuryan; Sergeï Kruglik; Nicolas Sandeau; Frédéric Louradour; Geneviève Bourg-Heckly
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Paper Abstract

We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.

Paper Details

Date Published: 22 February 2011
PDF: 6 pages
Proc. SPIE 7893, Endoscopic Microscopy VI, 789309 (22 February 2011); doi: 10.1117/12.875440
Show Author Affiliations
Donald A. Peyrot, Univ. Pierre et Marie Curie (France)
Claire Lefort, XLIM, CNRS, Univ. de Limoges (France)
D. Abi Haidar, Univ. Pierre et Marie Curie (France)
Tigran Mansuryan, XLIM, CNRS, Univ. de Limoges (France)
Sergeï Kruglik, Univ. Pierre et Marie Curie (France)
Nicolas Sandeau, Univ. Pierre et Marie Curie (France)
Frédéric Louradour, XLIM, CNRS, Univ. de Limoges (France)
Geneviève Bourg-Heckly, Univ. Pierre et Marie Curie (France)

Published in SPIE Proceedings Vol. 7893:
Endoscopic Microscopy VI
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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