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Proceedings Paper

Coherent anti-Stokes Raman scattering imaging through turbid medium
Author(s): Rajan Arora; Georgi I. Petrov; Vladislav V. Yakovlev
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Paper Abstract

Coherent Raman microspectroscopy imaging is an emerging technique for non-invasive, chemically-specific optical imaging. The wealth of information available through hyperspectral imaging potentially allows discriminating and imaging different chemicals in biological tissues, which is important, for example, for stain-free histopathology. One of the advantages of nonlinear Raman microspectroscopy is the ability to achieve high spatial resolution imaging in the presence of light scattering, when conventional confocal microscopy fails. In this report, a commercially available hyperspectral analysis software is utilized for analysis of vibrational spectra collected through highly scattering medium In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated.

Paper Details

Date Published: 28 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031B (28 February 2011); doi: 10.1117/12.875433
Show Author Affiliations
Rajan Arora, Univ. of Wisconsin-Milwaukee (United States)
Georgi I. Petrov, Univ. of Wisconsin-Milwaukee (United States)
Vladislav V. Yakovlev, Univ. of Wisconsin-Milwaukee (United States)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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