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Proceedings Paper

Ultrasound and photoacoustic imaging to monitor mesenchymal stem cells labeled with gold nanoparticles
Author(s): Seung Yun Nam; Laura M. Ricles; Konstantin Sokolov; Laura J. Suggs; Stanislav Y. Emelianov
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Paper Abstract

Mesenchymal stem cells (MSCs) are versatile in many tissue engineering applications and have the potential to be used for cellular therapies because they can differentiate into many cell types. Specifically, the use of MSCs for the treatment of ischemic disease is promising because MSCs can express characteristics of vascular cells. MSCs can promote vascular growth at the site of injury after delivery using a PEGylated fibrin gel. In order to quantitatively assess in vivo delivery and differentiation of MSCs, a non-invasive and high-resolution imaging technique is required. In this study, the combined ultrasound and photoacoustic imaging was demonstrated to monitor MSCs labeled with citrate-stabilized gold nanoparticles (Au NPs). It was observed that uptake of nanoparticles did not have a significant effect on cell viability and proliferation over a two-week period. Four different cell concentrations of either the non-labeled MSCs or the Au NP labeled MSCs were embedded in the tissue mimicking gelatin phantom. The ultrasound and photoacoustic signals were acquired and quantitatively analyzed to assess sensitivity and accuracy of the developed imaging approach. Furthermore, based on the results, the feasibility of in vivo ultrasound and photoacoustic imaging of MSCs was discussed.

Paper Details

Date Published: 18 February 2011
PDF: 7 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991Z (18 February 2011); doi: 10.1117/12.875262
Show Author Affiliations
Seung Yun Nam, The Univ. of Texas at Austin (United States)
Laura M. Ricles, The Univ. of Texas at Austin (United States)
Konstantin Sokolov, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
The Univ. of Texas at Austin (United States)
Laura J. Suggs, The Univ. of Texas at Austin (United States)
Stanislav Y. Emelianov, The Univ. of Texas at Austin (United States)
The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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