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Proceedings Paper

Three-dimensional polarization second harmonic generation (3D-PSHG) imaging: the effect of the tilted-off the plane SHG active structures
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Paper Abstract

Polarization sensitive second harmonic generation (PSHG) provides additional information in intensity only SHG imaging. In particular, it offers the SHG effective orientation of the implicated SHG active structures. Assuming that those structures possess cylindrical symmetry, the supplementary contrast is based on the ratio of two non-vanishing, independent elements of the χ2 tensor. This ratio is experimentally extracted by fitting pixel by pixel a theoretical model to the PSHG images and by finding the maximum frequency value or the mean of the consequent pixels' histogram. In the present study we show that the above χ2 elements' ratio critically depends on the tilted-off the plane SHG active structures. We performed PSHG in different z-planes of a starch granule (presenting radially oriented amylopectin molecules, the SHG source in starch) and we found different pick values of the χ2 elements' ratio histogram for each plane. By assuming a fixed value for the χ2 elements' ratio, we present here a generalized three dimensional (3D) model that determines the 3D orientation of the SHG active structures.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030H (11 February 2011); doi: 10.1117/12.875254
Show Author Affiliations
Sotiris Psilodimitrakopoulos, ICFO - Instituto de Cièncias Fotònicas (Spain)
Ivan Amat-Roldan, ICFO - Instituto de Cièncias Fotònicas (Spain)
David Artigas, ICFO - Instituto de Cièncias Fotònicas (Spain)
Univ. Politècnica de Catalunya (Spain)
Pablo Loza-Alvarez, ICFO - Instituto de Cièncias Fotònicas (Spain)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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