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Proceedings Paper

Broadband coherent Raman imaging for multiplexed detection
Author(s): Brad Littleton; Fred Festy; Simon Ameer-Beg; David Richards
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Paper Abstract

The Raman spectrum of a fluorescent chromophore typically has many spectral features, which differ markedly between dyes even if their electronic spectra are similar. This high information content makes it possible to distinguish biomarkers based on their Raman spectra. Coherent anti-Stokes Raman scattering may therefore allow for the simultaneous measurement of more biomarkers than is possible with fluorescent imaging, while avoiding bleaching and sample autofluorescence. We have built a broadband CARS microspectrometer to demonstrate the principle of CARS multiplexing and investigate the potential to apply the system to studies of biological samples.

Paper Details

Date Published: 28 February 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032J (28 February 2011); doi: 10.1117/12.875180
Show Author Affiliations
Brad Littleton, King's College London (United Kingdom)
Fred Festy, King's College London (United Kingdom)
Simon Ameer-Beg, King's College London (United Kingdom)
David Richards, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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