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Proceedings Paper

Combined OCT and CARS using a single ultrashort pulse Ti:Sapphire laser
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Paper Abstract

Optical coherence tomography (OCT) is a non-invasive in vivo biomedical imaging modality capable of three-dimensional visualization of tissue morphology permitting imaging at high speed and sensitivity. Coherent Anti- Stokes Raman Scattering (CARS) is a nonlinear spectroscopic technique which provides molecular information due to a four wave mixing process. In order to extend the performance of OCT towards detecting the molecular fingerprint of biological samples a combined CARS/OCT setup has been developed that employs only a single ultrashort pulse Ti:Sapphire laser which enables high axial resolution OCT and simultaneously combined with a spectral shaper a CARS setup. During first measurements the same area of a sample was imaged twice, applying OCT and CARS consecutively. OCT was used to perform three-dimensional morphological screening. Due to CARS additional chemical information could be gained for two dimensions. The spectrum was modified computer controlled to match the requirements for the generation of a CARS signal whereas for OCT the unmodified spectrum was applied. Fluids such as dimethylsulfoxide (DMSO) and PBS were compared in a cuvette to demonstrate the functionality of the multimodal setup. As a biological sample a 100 m thickcr oss section through a human optic nerve surrounded by sclera was investigated.

Paper Details

Date Published: 10 February 2011
PDF: 8 pages
Proc. SPIE 7892, Multimodal Biomedical Imaging VI, 78920B (10 February 2011); doi: 10.1117/12.875174
Show Author Affiliations
Claudia Hoffmann, Leibniz Univ. Hannover (Germany)
Bernd Hofer, Medical Univ. Vienna (Austria)
Cardiff Univ. (United Kingdom)
Angelika Unterhuber, Medical Univ. Vienna (Austria)
Cardiff Univ. (United Kingdom)
Boris Poavzay, Medical Univ. Vienna (Austria)
Cardiff Univ. (United Kingdom)
Uwe Morgner, Leibniz Univ. Hannover (Germany)
Laser Zentrum Hannover e.V. (Germany)
Wolfgang Drexler, Medical Univ. Vienna (Austria)
Cardiff Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 7892:
Multimodal Biomedical Imaging VI
Fred S. Azar; Xavier Intes, Editor(s)

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