Share Email Print
cover

Proceedings Paper

Magnetomotive optical coherence microscopy for cell dynamics and biomechanics
Author(s): Xing Liang; Benedikt W. Graf; Renu John; Vasilica Crecea; Freddy T Nguyen; Huafeng Ding; Hyon-min Song; Gabriel Popescu; Alexander Wei; Stephen A. Boppart
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Magnetomotive microscopy techniques are introduced to investigate cell dynamics and biomechanics. These techniques are based on magnetomotive transducers present in cells and optical coherence imaging techniques. In this study, magnetomotive transducers include magnetic nanoparticles (MNPs) and fluorescently labeled magnetic microspheres, while the optical coherence imaging techniques include integrated optical coherence (OCM)and multiphoton (MPM) microscopy,and diffraction phase microscopy (DPM). Samples used in this study are murine macrophage cells in culture that were incubated with magnetomotive transducers. MPMis used to visualize multifunctional microspheres based on their fluorescence, while magnetomotive OCM detects sinusoidal displacements of the sample induced by a magnetic field. DPM is used to image single cells at a lower frequency magnetic excitation, and with its Fourier transform light scattering (FTLS) analysis, oscillation amplitude is obtained, indicating the relative biomechanical properties of macrophage cells. These magnetomotive microscopy method shave potential to be used to image and measure cell dynamics and biomechanical properties. The ability to measure and understand biomechanical properties of cells and their microenvironments, especially for tumor cells, is of great importance and may provide insight for diagnostic and subsequently therapeutic interventions.

Paper Details

Date Published: 16 February 2011
PDF: 7 pages
Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 788926 (16 February 2011); doi: 10.1117/12.875139
Show Author Affiliations
Xing Liang, Univ. of Illinois at Urbana-Champaign (United States)
Benedikt W. Graf, Univ. of Illinois at Urbana-Champaign (United States)
Renu John, Univ. of Illinois at Urbana-Champaign (United States)
Vasilica Crecea, Univ. of Illinois at Urbana-Champaign (United States)
Freddy T Nguyen, Univ. of Illinois at Urbana-Champaign (United States)
Huafeng Ding, Univ. of Illinois at Urbana-Champaign (United States)
Hyon-min Song, Univ. of Illinois at Urbana-Champaign (United States)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)
Alexander Wei, Purdue Univ. (United States)
Stephen A. Boppart, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 7889:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top