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Proceedings Paper

Nanosurgery with near-infrared 12-femtosecond and picosecond laser pulses
Author(s): Aisada Uchugonova; Huijing Zhang; Cornelius Lemke; Karsten König
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Paper Abstract

Laser-assisted surgery based on multiphoton absorption of NIR laser light has great potential for high precision surgery at various depths within the cells and tissues. Clinical applications include refractive surgery (fs-LASIK). The non-contact laser method also supports contamination-free cell nanosurgery. Here we apply femtosecond laser scanning microscopes for sub-100 nm surgery of human cells and metaphase chromosomes. A mode-locked 85 MHz Ti:Sapphire laser with an M-shaped ultrabroad band spectrum (maxima: 770 nm/830 nm) with an in situ pulse duration at the target ranging from 12 femtoseconds up to 3 picoseconds was employed. The effects of laser nanoprocessing in cells and chromosomes have been quantified by atomic force microscopy (AFM) and electron microscopy. These studies demonstrate the potential of extreme ultrashort femtosecond laser pulses at low mean milliwatt powers for sub-100 nm surgery.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031N (11 February 2011); doi: 10.1117/12.875032
Show Author Affiliations
Aisada Uchugonova, Univ. des Saarlandes (Germany)
Univ. of California, San Diego (United States)
Huijing Zhang, Univ. des Saarlandes (Germany)
Cornelius Lemke, Univ. of Jena (Germany)
Karsten König, Univ. des Saarlandes (Germany)
Beckman Laser Institute (United States)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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