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Proceedings Paper

Multiparameter label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) with biological applications
Author(s): Charles H. Camp; Siva Yegnanarayanan; Ali A. Eftekhar; Ali Adibi
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Paper Abstract

Flow cytometry is an invaluable analytical tool that provides statistical information about sample populations. By optically probing samples at high-speed, flow cytometers retrieve information about basic morphology and composition. The optical elastic scatter is indicative of sample size and granularity while molecular information requires the addition of fluorescent labels, which have limitations such as spectral overlap, non-specific binding, and cellular toxicity. In this work, we present the first multiparameter label-free flow cytometer that observes the elastically forward-scattered light (FSC) and probes the intrinsic Raman vibrations of passing samples using multiplex coherent anti-Stokes Raman scattering (MCARS). MCARS, as a broadband technique, probes a large region of the Raman spectrum; thus, leading to rich molecularly-sensitive information. Additionally, we present the first experimental investigations of two biological systems using the multiparameter flow cytometer: Saccharomyces cerevisiae, a yeast often used as a model system for eukaryotic organisms, and Phaeodactylum tricornutum, a diatom under investigation for biofuel production.

Paper Details

Date Published: 11 February 2011
PDF: 11 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031G (11 February 2011); doi: 10.1117/12.874998
Show Author Affiliations
Charles H. Camp, Georgia Institute of Technology (United States)
Siva Yegnanarayanan, Georgia Institute of Technology (United States)
Ali A. Eftekhar, Georgia Institute of Technology (United States)
Ali Adibi, Georgia Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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