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Proceedings Paper

Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography
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Paper Abstract

In imaging of turbid biological samples using optical techniques, optical clearing methods can compensate for the lack of light penetration due to strong attenuation. The addition of optical clearing agents into scattering media increases the optical homogeneity of the sample and reduces its turbidity, allowing for the increased light penetration. In this study we investigated the extent of optical clearing in porcine skin by utilizing various concentrations of glucose solution. A goldplated mirror was fixed beneath the tissue and percentage clearing was determined by measuring the change in intensity of optical coherence tomography light returning from the mirror over time. A ratio of percentage clearing per tissue thickness for 10%, 30%, and 50% glucose was determined to be to be (4.7 ± 1.6%) mm-1 (n = 6), (10.6 ± 2.0%) mm-1 (n = 7), and (21.8 ± 2.2%) mm-1 (n = 5), respectively. Although the extent of optical clearing in porcine skin was more significant for 50% glucose, the osmotic stress on the sample can cause considerable morphology change, thus a suitable concentration must be chosen for particular circumstances.

Paper Details

Date Published: 12 February 2011
PDF: 6 pages
Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 78892A (12 February 2011); doi: 10.1117/12.874991
Show Author Affiliations
Narendran Sudheendran, Univ. of Houston (United States)
Mohamed Mohamed, Univ. of Houston (United States)
Mohamad G. Ghosn, Univ. of Houston (United States)
Baylor College of Medicine (United States)
Valery V. Tuchin, Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Russian Federation)
Kirill V. Larin, Univ. of Houston (United States)
Saratov State Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 7889:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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