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Proceedings Paper

Nano-LISA for in vitro diagnostic applications
Author(s): Saher Maswadi; Randolph D. Glickman; Rowe Elliott; Norman Barsalou
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Paper Abstract

We previously reported the detection of bacterial antigen with immunoaffinity reactions using laser optoacoustic spectroscopy and antibody-coupled gold nanorods (Ab-NR) as a contrast agent specifically targeted to the antigen of interest. The Nano-LISA (Nanoparticle Linked Immunosorbent Assay) method has been adapted to detect three very common blood-borne viral infectious agents, i.e. human T-lymphotropic virus (HTLV), human immunodeficiency virus (HIV) and hepatitis-B (Hep-B). These agents were used in a model test panel to illustrate the performance of the Nano-LISA technique. A working laboratory prototype of a Nano-LISA microplate reader-sensor was assembled and tested against the panel containing specific antigens of each of the infectious viral agents. Optoacoustic (OA) responses generated by the samples were detected using the probe beam deflection technique, an all-optical, non-contact technique. A LabView graphical user interface was developed for control of the instrument and real-time display of the test results. The detection limit of Nano-LISA is at least 1 ng/ml of viral antigen, and can reach 10 pg/ml, depending on the binding affinity of the specific detection antibody used to synthesize the Ab-NR. The method has sufficient specificity, i.e. the detection reagents do not cross-react with noncomplementary antigens. Thus, the OA microplate reader, incorporating NanoLISA, has adequate detection sensitivity and specificity for use in clinical in vitro diagnostic testing.

Paper Details

Date Published: 17 February 2011
PDF: 10 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991O (17 February 2011); doi: 10.1117/12.874922
Show Author Affiliations
Saher Maswadi, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Randolph D. Glickman, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Rowe Elliott, Naval Medical Research Unit - San Antonio (United States)
Norman Barsalou, Naval Medical Research Unit - San Antonio (United States)

Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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