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Proceedings Paper

Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?
Author(s): Yuan Liu; Baohong Yuan; Joseph Vignola
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Paper Abstract

The question of whether particle size affects modulation efficiency, defined as the ratio of ultrasound-modulated fluorescence (UMF) signal to DC (direct current) signal, of the fluorescence emission from four different sized fluorescent particles was investigated experimentally. The four particles are streptavidin-conjugated Alexa Fluo 647 (~5 nm in diameter) and three carboxylate-modified fluorescent microspheres (FM) with different diameters of 0.02, 0.2, and 1.0 μm. Modulation efficiency was evaluated as a function of the fluorophore size and fluorophore concentration. The modulation efficiency was improved about two times when the size of the fluorescent particles is increased from 5 nm to 1 μm. This result implies that using large fluorescence particles can slightly improve the modulation efficiency but the improvement is limited.

Paper Details

Date Published: 22 February 2011
PDF: 6 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789934 (22 February 2011); doi: 10.1117/12.874906
Show Author Affiliations
Yuan Liu, The Univ. of Texas at Arlington (United States)
Baohong Yuan, The Univ. of Texas at Arlington (United States)
Joseph Vignola, Catholic Univ. of America (United States)

Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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