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Proceedings Paper

Immunohistochemical analysis of immune response in breast cancer and melanoma patients after laser immunotherapy
Author(s): Robert E. Nordquist; Shelly L. Bishop; Halie Ferguson; Melville B. Vaughan; Jessnie Jose; Katherine Kastl; Long Nguyen; Xiaosong Li; Hong Liu; Wei R. Chen
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Paper Abstract

Laser immunotherapy (LIT) has shown great promise in pre-clinical studies and preliminary clinical trials. It could not only eradicate treated local tumors but also cause regression and elimination of untreated metastases at distant sites. Combining a selective photothermal therapy with an active immunological stimulation, LIT can induce systemic anti-tumor immune responses. Imiquimod (IMQ), a toll-like receptor agonist, was used for the treatment of late-stage melanoma patients and glycated chitosan (GC), a biological immunological modulator, was used for the treatment of late-stage breast cancer patients, in combination of irradiation of a near-infrared laser light. To observe the immunological changes before and after LIT treatment, the pathological tissues of melanoma and breast cancer patients were processed for immunohistochemical analysis. Our results show that LIT changed the expressions of several crucial T cell types. Specifically, we observed significant decreases of CD3+ T-cells and a significant increase of CD4+,CD8+, and CD68+ T-cells in the tumor samples after LIT treatment. While not conclusive, our study could shed light on one the possible mechanisms of anti-tumor immune responses induced by LIT. Further studies will be conducted to identify immunological biomarkers associated with LIT-induced clinical response.

Paper Details

Date Published: 23 February 2011
PDF: 10 pages
Proc. SPIE 7900, Biophotonics and Immune Responses VI, 790007 (23 February 2011); doi: 10.1117/12.874875
Show Author Affiliations
Robert E. Nordquist, ImmunoPhotonics Inc. (United States)
Wound Healing of Oklahoma, Inc. (United States)
Shelly L. Bishop, ImmunoPhotonics Inc. (United States)
Wound Healing of Oklahoma, Inc. (United States)
Halie Ferguson, Univ. of Central Oklahoma (United States)
Melville B. Vaughan, Univ. of Central Oklahoma (United States)
Jessnie Jose, Univ. of Central Oklahoma (United States)
Katherine Kastl, Univ. of Central Oklahoma (United States)
Long Nguyen, Univ. of Central Oklahoma (United States)
Xiaosong Li, Univ. of Central Oklahoma (United States)
First Affiliated Hospital of Chinese PLA General Hospital (China)
Chinese PLA General Hospital (China)
Hong Liu, Univ. of Oklahoma (United States)
Wei R. Chen, Univ. of Central Oklahoma (United States)


Published in SPIE Proceedings Vol. 7900:
Biophotonics and Immune Responses VI
Wei R. Chen, Editor(s)

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