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Proceedings Paper

Autofluorescence ratio imaging of human colonic adenomas
Author(s): Katsuichi Imaizumi; Yoshinori Harada; Naoki Wakabayashi; Yoshihisa Yamaoka; Ping Dai; Hideo Tanaka; Tetsuro Takamatsu
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Paper Abstract

Recently autofluorescence imaging (AFI) endoscopy, visualizing tissue fluorescence in combination with reflected light, has been adopted as a technique for detecting neoplasms in the colon and other organs. However, autofluorescence colonoscopy is not infallible, and improvement of the detection method can be expected to enhance the performance. Colonic mucosa contains metabolism-related fluorophores, such as reduced nicotinamide adenine dinucleotide, which may be useful for visualizing neoplasia in autofluorescence endoscopy. We examined sliced cross-sections of endoscopically resected tubular adenomas under a microscope. Fluorescence images acquired at 365-nm excitation (F365ex) and 405-nm excitation (F405ex), and reflectance images acquired at 550 nm (R550) were obtained. Fluorescence ratio (F365ex/F405ex) images and reflectance/fluorescence ratio (R550/F405ex) images were calculated from the acquired images. The fluorescence ratio images could distinguish adenomatous mucosa from normal mucosa more clearly than the reflectance/fluorescence ratio images. The results showed that the autofluorescence ratio imaging is a potential technique for increasing the diagnostic power of autofluorescence endoscopy.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020E (11 February 2011); doi: 10.1117/12.874869
Show Author Affiliations
Katsuichi Imaizumi, Kyoto Prefectural Univ. of Medicine (Japan)
Olympus Medical Systems Corp. (Japan)
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Naoki Wakabayashi, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshihisa Yamaoka, Kyoto Prefectural Univ. of Medicine (Japan)
Ping Dai, Kyoto Prefectural Univ. of Medicine (Japan)
Hideo Tanaka, Kyoto Prefectural Univ. of Medicine (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)


Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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