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Proceedings Paper

Confocal imaging at the nanoscale with two-color STED microscopy
Author(s): Hilmar Gugel; Arnold Giske; Marcus Dyba; Jochen Sieber
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Paper Abstract

STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to confocal imaging with biological specimens.

Paper Details

Date Published: 28 February 2011
PDF: 7 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050X (28 February 2011); doi: 10.1117/12.874779
Show Author Affiliations
Hilmar Gugel, Leica Microsystems CMS GmbH (Germany)
Arnold Giske, Leica Microsystems CMS GmbH (Germany)
Marcus Dyba, Leica Microsystems CMS GmbH (Germany)
Jochen Sieber, Leica Microsystems CMS GmbH (Germany)

Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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