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Proceedings Paper

Time-resolved confocal microscopy of cryogenic processes in biological tissues
Author(s): M. Schellenberg; T. Bergmann; E. Peev; M. Kloster; J. Napier; W. Neu
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Paper Abstract

Cryogenic procedures are fundamental tools in modern biology, e.g. for conservation or purification of biological materials. The processes occurring in biological cells and tissues during freezing and thawing are subject to ongoing research. Optimization of cell survival rates demands the development and evaluation of exactly defined temperature profiles. 4D-DMD-microscopy is capable of imaging these highly dynamic processes with high spatial and temporal resolution, utilizing well established staining procedures for differentiating structures of interest.

Paper Details

Date Published: 11 February 2011
PDF: 12 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79041F (11 February 2011); doi: 10.1117/12.874701
Show Author Affiliations
M. Schellenberg, Univ. of Applied Sciences Emden/Leer (Germany)
T. Bergmann, Univ. of Applifed Sciences Giessen-Friedberg (Germany)
E. Peev, Univ. of Applied Sciences Emden/Leer (Germany)
M. Kloster, Univ. of Applied Sciences Emden/Leer (Germany)
J. Napier, Univ. of Applied Sciences Emden/Leer (Germany)
W. Neu, Univ. of Applied Sciences Emden/Leer (Germany)


Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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