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Proceedings Paper

Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
Author(s): Yuan Luo; Ioannis Zervantonakis; Se Baek Oh; Roger Kamm; George Barbastathis
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Paper Abstract

A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.

Paper Details

Date Published: 22 February 2011
PDF: 5 pages
Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910C (22 February 2011); doi: 10.1117/12.874570
Show Author Affiliations
Yuan Luo, Massachusetts Institute of Technology (United States)
Ioannis Zervantonakis, Massachusetts Institute of Technology (United States)
Se Baek Oh, Massachusetts Institute of Technology (United States)
Roger Kamm, Massachusetts Institute of Technology (United States)
George Barbastathis, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7891:
Design and Quality for Biomedical Technologies IV
Ramesh Raghavachari; Rongguang Liang, Editor(s)

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