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Proceedings Paper

Chemical release from single-PMMA microparticles monitored by CARS microscopy
Author(s): Annika Enejder; Fredrik Svedberg; Lars Nordstierna; Magnus Nydén
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Paper Abstract

Microparticles loaded with antigens, proteins, DNA, fungicides, and other functional agents emerge as ideal vehicles for vaccine, drug delivery, genetic therapy, surface- and crop protection. The microscopic size of the particles and their collective large specific surface area enables highly active and localized release of the functional substance. In order to develop designs with release profiles optimized for the specific application, it is desirable to map the distribution of the active substance within the particle and how parameters such as size, material and morphology affect release rates at single particle level. Current imaging techniques are limited in resolution, sensitivity, image acquisition time, or sample treatment, excluding dynamic studies of active agents in microparticles. Here, we demonstrate that the combination of CARS and THG microscopy can successfully be used, by mapping the spatial distribution and release rates of the fungicide and food preservative IPBC from different designs of PMMA microparticles at single-particle level. By fitting a radial diffusion model to the experimental data, single particle diffusion coefficients can be determined. We show that release rates are highly dependent on the size and morphology of the particles. Hence, CARS and THG microscopy provides adequate sensitivity and spatial resolution for quantitative studies on how singleparticle properties affect the diffusion of active agents at microscopic level. This will aid the design of innovative microencapsulating systems for controlled release.

Paper Details

Date Published: 22 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030S (22 February 2011); doi: 10.1117/12.874537
Show Author Affiliations
Annika Enejder, Chalmers Univ. of Technology (Sweden)
Fredrik Svedberg, Chalmers Univ. of Technology (Sweden)
Lars Nordstierna, Chalmers Univ. of Technology (Sweden)
Magnus Nydén, Chalmers Univ. of Technology (Sweden)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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